Anti-JPH3 Antibody (C15-1317-622)

General description

Junctophilin-3 (JPH3, JP-3), also known as Trinucleotide repeat-containing gene 22 protein (TNRC22), is a single-pass type IV membrane protein belonging to the junctophilin family. Junctophilins are important components of the junctional complexes which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. JPH3 is specifically expressed in brain. A mutation associated with JPH3 is the cause of Huntington disease-like type 2 (HDL2), a neurodegenerative disorder.

Specificity

This antibody recognizes the C-terminus of JPH3.

Immunogen

Epitope: Near C-terminus.

KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human JPH3.

Application

Anti-JPH3 Antibody is a highly specific rabbit polyclonal antibody, that targets Junctophilin-3 & has been tested in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected JPH3 in human brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected JPH3 in human brain cells.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Quality

Evaluated by Western Blotting/Peptide Inhibition Assay in Daudi cell lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected JPH3 in 15 µg of Daudi cell lysate.

Target description

~105 kDa observed. Uniprot describes 2 isoforms at ~20 kDa and ~81 kDa Uncharacterized bands may appear in some lysates.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Daudi cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: Q8WXH2. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... JPH3(57338). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.