Anti-KIF5A Antibody (C15-1317-527)

General description

Kinesin heavy chain isoform 5A (KIF5) is a member of the kinesin-like protein family. These proteins are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5A performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5A was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the isoforms of KIF5 (KIF5A) is thought to be involved in hereditary spastic paraplegias.

Specificity

This antibody can recognize isoforms KIF5A, KIF5B, and KIF5C.

Immunogen

KLH-conjugated linear peptide corresponding to human KIF5A.

Application

Detect the KIF5A protein using this Anti-KIF5A validated for use in WB & ICC.

Immunocytochemistry Analysis: 5 µg/mL from a representative lot detected KIF5A in K562 cells.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blot in mouse brain nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected KIF5A in 15 µg/mL of mouse brain nuclear extract.

Target description

~117 kDa observed. Uncharacterized bands may appear at ~45 kDa and ~57 kDa in some lysates.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain nuclear extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human. technique(s): immunocytochemistry: suitable, western blot: suitable. UniProt accession no.: Q12840. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... KIF5A(3798). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.