Anti-MAGL Antibody (C15-1317-318)

General description

Monoglyceride lipase (MGL), or alternatively HU-K5, Lysophospholipase homolog, Lysophospholipase-like, or Monoacylglycerol lipase (MAGL) is a protein encoded by the MGLL gene in humans and is very important in lipid metabolism. Monoglyceride lipase is the enzyme that converts monoacylglycerides (key building blocks of lipids) into free fatty acid chains and glycerol. Also, Monoglyceride Lipase hydrolyzes endocannabinoids which ultimately can regulate nociperception and the perception of pain, so the enzyme is being studied in pain mediation therapies. Monoglyceride Lipase is expressed in many tissues including fat, lung, liver, brain and heart. In disease, Monoglyceride Lipase is being studied most intensely in cancer research. In some cancers it appears to be play a suppressive role in regulating AKT mediated signaling, but in others, since the enzyme regulates the levels of fatty acids that can serve as intra and intercellular signaling molecules, Monoglyceride lipase activity seems to promote cancer cell migration, invasion and growth.

Immunogen

Recombinant protein corresponding to mouse MAGL.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MAGL in human cerebral cortex tissue.
Immunohistochemistry Analysis: A representative lot detected MAGL in human hippocampus tissue (Mulder, J., et al. (2011). Brain. 134:1041-1060).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-MAGL Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of MAGL.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MAGL in 10 µg of mouse brain tissue lysate.

Target description

~ 31/33 kDa observed. This protein can be alternatively spliced, so western blots may show a doublet. Evidence for alternative splicing of MAGL, can run as doublet, ~31 and ~33 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse, rat. technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_001159721. UniProt accession no.: O35678. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... MGLL(11343). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.