Specificity
Recognizes MARCKS.
Immunogen
Full length recombinant rat MARCKS.
Application
Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.
Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.
Optimal working dilutions must be determined by the end user.
Linkage
Replaces: AB5427
Physical form
Format: Purified
Semi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide.
Analysis Note
Control
POSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat. manufacturer/tradename: Chemicon®. . technique(s): western blot: suitable. NCBI accession no.: NM_002356.5. UniProt accession no.: P29966. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... MARCKS(4082). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB9298
