ANTI-MARCKS ANTIBODY COMPLETE SEQUENCE (C005B-240319)

Specificity

Recognizes MARCKS.

Immunogen

Full length recombinant rat MARCKS.

Application

Anti-MARCKS Antibody, Complete sequence is an antibody against MARCKS for use in WB.

Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 70-85 kDa (depending on species) MARCKS protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 8%. Overnight incubation with the antibody is recommended.

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: AB5427

Physical form

Format: Purified

Semi-purified immunoglobulin. Liquid in 120 mM NaCl, 25 mM Tris-HCl, pH 7.3 containing 0.01% sodium azide.

Analysis Note

Control
POSITIVE CONTROL: Neonatal rat brain extract. MARCKS is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze MARCKS content by immunoblot.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. NCBI accession no.: NM_002356.5. UniProt accession no.: P29966. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... MARCKS(4082). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:

41116126

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

50

Unit of Measurement:

UL

MPN:

AB9298

Temperature Control Device:

Yes