ANTI-MCM7 ANTIBODY (C15-1316-662)

General description

MCM7 is a highly conserved mini-chromosome maintenance protein (MCM) that is involved in the initiation of eukaryotic genome replication. It forms a hexameric complex with MCM4 and MCM6 (two subunits of MCM7, two subunits of MCM4 and two subunits of MCM7) and this complex regulates the helicase activity of the pre-replication complex. This complex is also involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. MCM7 is reported to bind to DNA in metaphase II in oocytes and remains associated with the DNA until the S-phase. Two isoforms of MCM7 have been reported, 61 kDa and 90 kDa. In neuroblastoma tumors MCM7 expression is shown to correlates with MYCN (v-myc myelocytomatosis viral related oncogene, neuroblastoma derived) amplification where in MCM7 is rapidly up-regulated by Inducible MYCN Expression. MYCN protein is shown to bind specifically to the MCM7 promoter region, which possesses an E-box binding site for the Myc oncoproteins.

Specificity

Other homologies: Mouse (71% sequence homology).

This antibody recognizes the N-terminus of MCM7

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human MCM7.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This MCM7 antibody is validated for use in WB for the detection of the MCM7 protein.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected MCM7 in 10 µg of L6 cell lysate.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MCM7 in 10 µg of HeLa cell lysate.

Target description

~82 kDa observed

Linkage

Replaces: MABE188

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, rat. species reactivity (predicted by homology): rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_005907. UniProt accession no.: P33993. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... MCM7(4176). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.