ANTI-MUC13 ANTIBODY CLONE 2E11.1

General description

Mucin-13 (MUC-13) is also called Down-regulated in colon cancer 1 (DRCC1). MUC-13 is an epithelial and hemopoietic transmembrane mucin that is likely involved in cell signaling. MUC-13 is highly expressed in epithelial tissues (especially in gastrointestinal and respiratory tracts) as well as kidney, small intestine, appendix and stomach.

Immunogen

Epitope: This antibody recognizes the cytoplasmic domain within the Beta subunit of MUC-13.

GST-tagged recombinant protein corresponding to the cytoplasmic domain of human MUC-13.

Application

Detect Mucin using this mouse monoclonal antibody, Anti-MUC13 Antibody, clone 2E11.1 validated for use in western blotting & IHC (Paraffin).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected MUC-13 in 10 µg of human kidney tissue lysate.

Immunohistochemistry Analysis: a 1:500 dilution from a representative lot detected MUC-13 in human ilium tissues.

Quality

Evaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MUC-13 in 10 µg of human small intestine tissue lysate.

Target description

~33 and ~60 kDa observed. Swiss-Prot describes a molecular weight of ~55 kDa Due post-translational modification, this protein is cleaved resulting in beta subunit (~33 kDa) and an alpha subunit (Williams, S. J., et al. (2001). J Biol Chem. 276(21):18327-18336. Maher, D. M., et al. (2011). Mol Cancer Res. 9(5):531-537.). Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.