General description
NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
Immunogen
KLH-conjugated linear peptide corresponding to human NENF.
Application
Detect the NENF protein using this Anti-NENF validated for use in WB & IHC.
Immunohistochemistry Analysis: 10 µg/mL of this antibody from a representative lot detected NENF in rat kidney tissue.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Quality
Evaluated by Western Blot in human kidney tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected NENF in 15 µg of human kidney tissue lysate.
Target description
~37 kDa observed
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human kidney tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human. technique(s): immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: Q9UMX5. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NENF(29937). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51202401
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABN352