Anti-NLRP6 (NALP6) Antibody (C15-1317-305)

General description

NRLP6 (NALP6) (also known as PYRIN-containing Apaf-1-like protein 5 or PYPAF5) belongs to a family of proteins that play a critical role in apoptosis and in inflammatory signaling. Its expression is highly restricted to granulocytes and T-cells. Co-expression of NALP6 with ASC results in synergistic activation of caspase-1-dependent cytokine processing, subsequent NF-kB activation, and downstream recruitment of PYPAF5 to punctate structures in the cytoplasm. Overlapping genes in NALP6/PYPAF5 locus encode two V2-type vasopressin isoreceptors: angiotensin-vasopressin receptor (AVR) and non-AVR.

Specificity

Other homologies: Mouse (93% sequence homology).

This antibody recognizes the NACHT domain of NLRP6 (NALP6).

Immunogen

Epitope: NACHT domain

Linear peptide corresponding to NACHT domain of human NLRP6 (NALP6).

Application

Anti-NLRP6 (NALP6) Antibody detects level of NLRP6 (NALP6) & has been published & validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NLRP6 (NALP6) in epithelial tissue of proximal and distal tubules of human kidney, and in human bone marrow tissue.

Quality

Evaluated by Western Blot in rat basophilic leukemia (RBL) cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected NLRP6 (NALP6) in 10 µg of rat basophilic leukemia (RBL) cell lysate.

Target description

~99 kDa observed

Analysis Note

Control
Rat basophilic leukemia (RBL) cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human. species reactivity (predicted by homology): rhesus macaque (based on 100% sequence homology). technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_612202. UniProt accession no.: P59044. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NLRP6(171389). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.