Anti-NXF1 Antibody (C15-1317-230)

General description

Nuclear RNA export factor 1 (also known as Tap protein) is encoded by the NXF1 gene. The protein expressed by this gene is one member of a family of nuclear RNA export factor gene. The Nuclear RNA export factor 1 protein is involved in export of mRNA species bearing retroviral constitutive transport elements (CTE) from the nucleus as well as in the export of mRNA from the nucleus to the cytoplasm. This protein is localized predominantly in the nucleoplasm at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. However, this protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A) RNA. This protein is present in the nuclear Y14 complex and has been shown to interact with Aly/REF.

Immunogen

Recombinant protein corresponding to human NXF1.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-NXF1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect NXF1 also known as Nuclear RNA export factor 1, Tip-associated protein.

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected NXF1 on 10 µg of A431 cell lysate.

Target description

~72 kDa observed. Two isoforms at 70 and 40 kDa have been described. Uncharacterized bands at ~48 and 100 kDa have been observed in some lysates.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
A431 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, rat, human. technique(s): western blot: suitable. NCBI accession no.: NP_006353. UniProt accession no.: Q9UBU9. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NXF1(10482). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.