ANTI-PCNA CLONE 3B22 ZOOMABRABBIT MONOCLONAL (C005B-261839)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 3B22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Proliferating cell nuclear antigen (PCNA). It targets an epitope within the first 150 amino acids.

Immunogen

GST/His-tagged recombinant fragment corresponding to the first 150 amino acids from the N-terminal region of human Proliferating cell nuclear antigen (PCNA).

Application

Anti-PCNA, clone 3B22 ZooMAb, Cat. No. ZRB1442, is a recombinant Rabbit monoclonal antibody that specifically targets PCNA and is tested for in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PCNA in HeLa cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PCNA in HeLa, A431, HUVEC cell lines.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PCNA in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant PCNA with a KD of 2.0 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform (UniProt: P48454; also known as EC:3.1.3.16, CAM-PRP catalytic subunit, Calcineurin, testis-specific catalytic subunit, Calmodulin-dependent calcineurin A subunit gamma isoform, PPP3CC) is encoded by the PPP3CC (also known as CALNA3, CNA3) gene (Gene ID: 5533) in human. PPP3CC is a calcium-dependent, calmodulin stimulated protein phosphatase that plays an essential role in the transduction of intracellular Ca2+-mediated signals. It is activated by Ca2+-bound calmodulin following an increase in intracellular Ca2+. At low Ca2+ concentrations, the catalytic subunit (aa 52-343; calcineurin A) is inactive and is bound to the regulatory subunit (calcineurin B) in which only two high-affinity binding sites are occupied by Ca2+. In response to elevated calcium levels, the occupancy of the low-affinity sites on calcineurin B by Ca2+ causes a conformational change of the C-terminal regulatory domain of calcineurin A, resulting in the exposure of the calmodulin-binding domain (aa 386-400) and in the partial activation of calcineurin A. The subsequent binding of Ca2+-bound calmodulin leads to the displacement of the autoinhibitory domain (aa 451-489) from the active site and possibly of the autoinhibitory segment (aa 401-408) from the substrate binding site which fully activates calcineurin A. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.