Anti-Ptx1 Antibody (C15-1317-240)

General description

Pituitary homeobox 1 (Ptx1; also known as Paired-like homeodomain transcription factor 1, Pituitary homeobox 1, or Hindlimb-expressed homeobox protein backfoot) is a nuclear transcription factor encoded by the Pitx1 (also known as Bft, Potx, Ptx1) gene in mouse. Ptx1 is reported to stimulate LHbeta gene promoter activity either alone or synergistically with the orphan nuclear receptor steroidogenic factor-1 (SF-1) and the early growth response gene 1 (Egr-1). Ptx1 is expressed in the hindlimb, but not the forelimb, of developing mouse embryos, and reduced hindlimb size and loss of hindlimb-specific features are seen in Pitx1-knockout mice.

Immunogen

Epitope: Near N-terminus

MBP-tagged recombinant protein corresponding to mouse Ptx1 near the N-terminus.

Application

Anti-Ptx1 Antibody is an antibody against Ptx1 for use in Western Blotting, Immunohistochemistry, Immunocytochemistry and Chromatin Immunoprecipitation (ChIP).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: A 1:250 dilution from a representative lot detected Ptx1 in 10 µg of GH3 cell lysate.
Immunohistochemistry Analysis: A representative lot detected Ptx1 in adult and developing pituitary (Lanctot, C., et al. (1999) Endocrinology. 140(3):1416-1422).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Ptx1 in AtT-20 cells (Langlais, D., et al. (2011). Molecular Endocrinology. 25(2): 348–359).

Quality

Evaluated by Western Blotting in αT3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ptx1 in 100 µg of αT3 cell lysate.

Target description

~48 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat. technique(s): ChIP: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_035227. UniProt accession no.: P70314. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Pitx1(18740)
rat ... Pitx1(113983). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.