ANTI-SEMAPHORIN 4A CLONE 8B5.1

General description

Semaphorin-4A (UniProt Q9H3S1; also known as Sema B, Semaphorin-B) is encoded by the SEMA4A (also known as CORD10, RP35, SEMAB, SEMB) gene (ORP UNQ783/PRO1317; Gene ID 64218) in human. Semaphorin-4A is a membrane-type class IV semaphorin originally characterized for its role in axon guidance during neural development, Semaphorin-4A also plays a role in Th cells activation and the differentiation of Th1 and Th17 cells. In addition Semaphorin-4A is involved in angiogenesis and endothelial cells migration by modulating VEGF signaling. Consistent with its important role in Th cells, Semaphorin-4A level is reported to be elevated in the serum samples from patients with multiple sclerosis (MS) respond poorly to interferon-beta (IFN-β) therapy. Semaphorin-4A neutralizing antibodies are reported to inhibit antigen-specific T cell generation and ameliorate experimental autoimmune encephalomyelitis (EAE), an animal model of MS.

Specificity

Expected to react with both spliced isoforms.

Immunogen

Epitope: Sema domain

GST-tagged recombinant protein corresponding to the Sema domain of human Semaphorin 4A.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Semaphorin 4A Antibody, clone 8B5.1 is validated for use in Western Blotting for the detection of Semaphorin 4A.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Semaphorin 4A in 10 µg of human retinal pigment epithelia (RPE) ARPE-19.

Quality

Evaluated by Western Blotting in human monocytic leukemia THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Semaphorin 4A in 10 µg of human monocytic leukemia THP-1 cell lysate.

Target description

~85 kDa observed. 83.6 kDa (isoform 1) and 69.1 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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UPC:

51434603

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

MPN:

MABN841

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes