Anti-Tet2 Antibody (C15-1317-133)

General description

Methylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS).

Immunogen

KLH-conjugated linear peptide corresponding to human Tet2.

Application

Anti-Tet2 detects levels of Tet2 proteins & has been published & validated for use in WB, IHC & ICC.

Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected Tet2 in rat medulla tissue.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Tet2 in human H9 Embryonic Stem cells.

Quality

Evaluated by Western Blotting in mouse Embryonic Stem cells.

Western Blotting Analysis: 1 µg/mL of this antibody detected Tet2 in 10 µg of mouse Embryonic Stem cells (Note: Isoforms 2 and 3 may observed at low concentrations or longer exposure times, while isoform 1 may be detected at higher concentrations or longer exposure times in certain cell lysates.).

Target description

~140 kDa observed. Three isoforms at 223 kDa (isoform 1), 130 kDa (isoform 2), and 133 kDa (isoform 3) are known to exist. This antibody should recognize all three isoforms based on sequence homology. An uncharacterized band may be observed at ~36 kDa in some cell lysates. (Note: Isoforms 2 and 3 may observed at low concentrations, while isoform 1 may be detected at higher concentrations in certain cell lysates.)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human, rat. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_060098. UniProt accession no.: Q6N021. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... TET2(54790). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.