General description
TFAM, also called mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development.
Immunogen
His-tagged recombinant protein corresponding to mouse mtTFA.
Application
Detect TFAM using this rabbit polyclonal antibody, Anti-TFAM Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TFAM in C2C12 cell lysate (Prof. B Kaufman, University of Pennsylvania.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Quality
Evaluated by Western Blotting in C2C12 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFAM in 10 µg of C2C12 cell lysate.
Target description
~27 kDa observed
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse, rat. technique(s): immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_033386. UniProt accession no.: P40630. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... TFAM(7019)mouse ... Tfam(21780). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.
- UPC:
- 51203605
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABE483
