ANTI-TRIMETHYL HISTONE H3(LYS9) ANTIBODY CLONE CMA308

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.

Specificity

Broad species cross-reactivity is expected, based on sequence homology.

This antibody recognizes Histone H3 trimethylated at Lys9. Some cross-reactivity with dimethyl Lys9 is detected at higher concentrations, but the difference in specificity is >27-fold1. Phosphorylation of Ser10 interferes with antibody binding to trimethyl Lys91.

Immunogen

Epitope: Trimethyl Lys9

Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, trimethylated on Lys9, conjugated to KLH.

Application

Anti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308 is a mouse monoclonal antibody for detection of Trimethyl Histone H3(Lys9) also known as H3K9me3, Histone H3 (tri methyl K9) & has been validated in WB, ELISA, ICC, IP, Mplex.

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1

Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for IP.1

Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

Western Blot Analysis:
A 0.5 – 2 μg/mL dilution of this antibody detected histone H3 in 10 μg of HeLa acid extract.

Target description

~17 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa Acid extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.