ANTI-VILLUS CRYPT. CLONE E5D10 (C005B-448816)

Specificity

Clone IF3F11 is a mouse monoclonal antibody that detects Adenine nucleotide translocator 1 (ANT1/SLC25A4). It targets an epitope within 15 amino acids from the internal region.

Immunogen

His-tagged, full-length, recombinant human ADP/ATP translocase 1 (ANT1).

Application

Quality Control Testing

Evaluated by Western Blotting in Human heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ANT1/SLC25A4 in Human heart tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected ANT1/SLC25A4 in Human skeletal muscle and Human brain tissue extracts.

Western Blotting Analysis: A representative lot detected ANT1/SLC25A4 in Western Blotting applications (Lu, Y-W., et al. (2017). Mol Biol Cell. 28(11):1489-1500; Acoba, M.G., et al. (2021). Cell Rep. 34(11):108869).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

ADP/ATP translocase 1 (UniProt: P12235; also known as ADP/ATP carrier protein 1, ADP/ATP carrier protein, heart/skeletal muscle isoform T1 1 , Adenine nucleotide translocator 1, ANT 1, Solute carrier family 25 member 4) is encoded by the SLC25A4 (also known as AAC1, ANT1) gene (Gene ID: 291) in human. ANT1 is a multi-pass inner mitochondrial membrane protein with six transmembrane domains. It serves as an ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to provide energy for cellular functions. It is reported to cycle between the cytoplasmic-open state (c-state) and the matrix-open state (m-state) and operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic or matrix side of the inner mitochondrial membrane. It is also involved in mitochondrial uncoupling and mitochondrial permeability transition pore activity. ANT also acts as a regulator of mitophagy independently of ADP:ATP antiporter activity. It promotes mitophagy via interaction with TIMM44 that leads to inhibition of presequence translocase TIMM23, thereby promoting stabilization of PINK1. ANT1 deficiency has been implicated in various pathological states, including hypertrophic cardiomyopathy, mitochondrial myopathy, lactic acidosis, and progressive external opthalmoplegia. (Ref.: Hoshino, A., et al. (2019). Nature. 575(7782); 375-379; Lu, Y-W., et al. (2017). Mol. Biol. Cell. 28(11):1489-1506; Kawamaoto, H., et al. (2011). Hum. Mol. Genet. 20(15); 2964-2974).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

UPC:

41181805

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

MPN:

MABS2267-100UL

Controlled Substances and Cyanide:

Yes