APN-Azide (C007B-605358)

APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.Standard protein labeling procedure (cysteine labeling)1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).3. Incubate at room temperature for 2 hours.4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.*Note: avoid thiol-containing buffers.

Specifications & Purity: ≥95%

Molecular Formula: C9H4N4

Molecular Weight: 168.15

More Information: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/A467144-SCI_382fd36b1518045b91c73d5740c786e7.pdf

UPC:

51113201

Condition:

New

Weight:

1.06 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

A467144-10mg

CAS:

1643841-88-6

Product Size:

10mg

Hazard Statement Codes:

H228;H315;H319;H335

Precautionary Statement Codes:

P210;P305+P351+P338;P370+P378