B12.F8

Cell Line Origin

Human anti-hepatitis C virus IgG1 secreting B cell

Cell Line Description

Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific to the native HCV nucleoprotein N3. This antibody binds to native HCV nucleoprotein with no observable reactivity for the NS3, NS4 and NS5 regions. This antibody identifies a major B cell epitope within the immunodominant nucleoprotein amino terminal subregion (aa. 34-45). B12.F8 can be used in immunochemistry, HCV protein purification and HCV typing.CM3.B6 (Sigma product number 11060101) and B12.F8 were derived from the same patient as confirmed by STR profiling.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16

Culture Medium

RPMI 1640 (R0883) + 2 mM L-L-Glutamine (G7513) + 1% NEAA (M7145) + 1% NaP (S8636) + 10% FBS / FCS (F2442) + 20U/ml Interleukin-6 (IL-6).

Subculture Routine

Maintain cultures between 3-9 x 10⁵/ml. 5% CO₂; 37 °C. Media change every four days. Saturation density 1.1 x 10⁶/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:

41181902

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

11060102-1VL

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes