B82 (HAMPRECHT)

Cell Line Origin

Mouse Fibroblast (L cells)

Cell Line Description

An 8-bromodeoxyuridine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). B82 lacks thymidine kinase and was isolated by virtue of resistance to 8-bromodeoxyuridine (30 μg/ml). ECACC has two catalogue entries for the B82 cell line which differ in that they were deposited by different groups: B82 (Sigma Catalogue number 85011408) and B82(Hamprecht) (Sigma Catalogue number 08062522). Several other cell lines in the ECACC catalogue have been derived from B82(Hamprecht).

Application

Used in the generation of cell hybrids.

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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UPC:

41106514

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VL

MPN:

08062522-1VL

Temperature Control Device:

Yes