Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The monoclonal antibody BC9 detects bovine tumour necrosis factor (TNF) in a radioimmunoassay with a detection limit of 24 pg/ml. Bovine recombinant TNF can be neutralised in vitro by addition of BC9. Cytotoxic assays showed a neutralising effect using L929 and WEHI 164 clone 13 cells.
Immunogen
Bovine recombinant TNF-alpha
Application
Detection of TNF in bioassay or radioimmunoassay, in vivo studies (neutralisation of TNF).
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 5-6x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-TNF, bovine. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51282606
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95030301-1VL
