Sigma-Aldrich
BCIP(R)/NBT-BLUE LIQUID SUBSTRATE SYSTEM FOR MEMBRANES
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan
Description
General description
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.
Application
BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.
Caution
Stable at least one year at 2-8 °C. This substrate is light-sensitive.
Other Notes
Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
Specifications
- UPC:
- 51201825
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- Quantity:
- 100
- Unit of Measurement:
- ML
- MPN:
- B3804-100ML
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