BCIP(R)/NBT-PURPLE LIQUID SUBSTRATE SYSTEM FOR MEMBRANES

Application

BCIP^®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase

• for the detection of secondary antibodies by immunoblot
• in ELISPOT assay
• in dot blot analysis
• in western blot to visualize protein bands on nitrocellulose membrane

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.

Caution

Stable at least one year at 2-8 °C.

Physical form

Ready-to-use.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

UPC:

41106607

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

100

Unit of Measurement:

ML

MPN:

B3679-100ML