BIOTIN FLUORESCEIN CONJUGATE (C005B-227397)

General description

It can be used for the detection of avidin and streptavidin.

Application

EIA (1:1,000)

Tissue Staining (1:50 - 1:100)

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Other Notes

Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203

Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

form: lyophilized solid. Quality Level: 100. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, protect from light. color: orange. solubility: DMF: 5 . mg/mL, DMSO: 5 . mg/mL, methanol: 5 . mg/mL. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

UPC:

12352200

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

MG

MPN:

343222-1MG