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Sigma-Aldrich

DMT-BIOTIN AMIDITE 0.1G AB SINGLE (C005B-455142)

General description Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′: hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis.

Description

General description

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

  • Behaves like any standard phosphoramidite on the DNA synthesizer
  • Readily soluble in acetonitrile
  • Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
  • A long, water-compatible linker arm ensures maximum sensitivity
  • Provides a coupling efficiency of at least 95%The product is configured for ABI Synthesizers

Legal Information

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

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Specifications
UPC:
41116133
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
Yes
Quantity:
1
Unit of Measurement:
GM
MPN:
M042030-0.1G
CAS:
1217500-22-5
Temperature Control Device:
Yes
Controlled Substances and Cyanide:
Yes
Catalog No. C005B-455142
Price: $324.13
List Price: $360.14
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