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Sigma-Aldrich

BIS-TRIS

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.

Description

General description

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.

Application

Bis-Tris has been used :

  • as a buffer for Electrophoresis
  • as a component of transfer buffer for Western Blotting

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

Other Notes

Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.

Specifications
UPC:
51171713
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
1
Unit of Measurement:
KG
MPN:
B9754-1KG
CAS:
6976-37-0
Catalog No. C005B-057848
Price: $1,232.68
List Price: $1,369.65
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