Sigma-Aldrich

BLEOMYCIN SULFATE (C005B-089264)

Name: BLEOMYCIN SULFATE (C005B-089264)
SKU: C005B-089264
Price: 1831.81 USD
Availability: InStock

Bleomycin Sulfate is an antineoplastic antibiotic isolated from Streptomyces verticillus . It is recommended for use as a cell culture agent at 10-100 μg/mL.

Description

General description

Bleomycin Sulfate is an antineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 μg/mL.

Chemical structure: glycopeptide

Application

Bleomycin Sulfate is an atineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 μg/mL.

Bleomycin sulfate from Streptomyces verticillus has been used:

to study the impact of telomere shortening in lung fibrosis
to induce lung injury and study its effect on CEACAM6 expression
as a DNA-alkylating agent to induce lung injury
study its effect on CEACAM6 expression
to cause lung injury
subsequent fibrosis in animals

Biochem/physiol Actions

Bleomycin sulfate binds to DNA, causes ssDNA scission at specific base sequences and inhibits DNA synthesis. This inhibitory action requires bleomycin to bind oxygen and a metal ion. It can also cleave RNA, to a lesser degree but more selectively. It acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis.

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Components

This product is a mixture of glycopeptides antibiotics containing Bleomycin A₂ (70%) and B₂(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.

Caution

This product should be stored at 2-8°C.

Preparation Note

The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.