Brain Heart Infusion Broth with 6.5% NaCl, 500 g

Brain Heart Infusion Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). Brain Heart Infusion Broth with 6.5 % NaCl is employed for the selective cultivation of salt tolerant microorganisms. High concentration of sodium chloride acts as a differential and/or selective agent by interfering with membrane permeability and osmotic and electro kinetic equilibrium in salt intolerant organisms. Proteose peptone and infusions (calf brain and beef heart) serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium. Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. Reference: 1. Rosenow, 1919, J. Dental Research, 1:205.