BT57 Reagent (C008B-268685)

BT57 is an all-in-one reagent for rapid denaturation, reduction and alkylation. In biopharmaceutical and proteomics research, native proteins with three-dimensional structures undergo denaturation, reduction, alkylation, desalting, and subsequent enzymatic digestion. Traditional denaturants such as urea and guanidine hydrochloride can cause chemical modifications to proteins, such as carbamylation. In addition, conventional alkylating reagents require protection from light. BT57 all-in-one reagent enables one-step addition and rapidly completes protein denaturation, reduction and alkylation. It is suitable for proteomics, biopharmaceutical and single-cell sample research. Physical Form: Lyophilized powder containing 1.5mg BT Surfactant, TCEP, ABC and CAA. Reconstitution: Reconstitute with 52 μL of ultrapure water. pH Range: pH 7–9 for protein denaturation. The reagent degrades and precipitates in solution at pH 2–4. Usage: Add 10 μL of BT57 reagent to 10 μL (50–100 μg) protein sample, mix well. Incubate in a dry bath at 60 °C for 20 min, or at 95 °C for 10 min.

Specifications & Purity: BT57 is an all-in-one reagent for rapid denaturation, reduction and alkylation. In biopharmaceutical and proteomics research, native proteins with three-dimensional structures undergo denaturation, reduction, alkylation, desalting, and subsequent enzymatic digestion. Traditional denaturants such as urea and guanidine hydrochloride can cause chemical modifications to proteins, such as carbamylation. In addition, conventional alkylating reagents require protection from light. BT57 all-in-one reagent enables one-step addition and rapidly completes protein denaturation, reduction and alkylation. It is suitable for proteomics, biopharmaceutical and single-cell sample research. Physical Form: Lyophilized powder containing 1.5mg BT Surfactant, TCEP, ABC and CAA. Reconstitution: Reconstitute with 52 μL of ultrapure water. pH Range: pH 7–9 for protein denaturation. The reagent degrades and precipitates in solution at pH 2–4. Usage: Add 10 μL of BT57 reagent to 10 μL (50–100 μg) protein sample, mix well. Incubate in a dry bath at 60 °C for 20 min, or at 95 °C for 10 min.

Grade: BioReagent;Suitable for mass spectrometry (MS)

Storage Temperature: Store at -20°C

Shipped in: Ice chest + Ice pads

UPC:

51442721

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

B1523260-1.5mg

Country of Origin:

China

Product Size:

1.5mg

Harmonized Tariff Code:

2931.90.90