BTO-1 (C008B-268528)

BTO-1 is a cell-permeable benzothiazolo-N-oxide compound that targets the ATP-binding pocket of polo-like kinase and is shown to inhibit Plk1 kinase activity in cell-free kinase assays (IC|50|= 8.0 μM) and suppress the phosphorylation of cellular Plk1 substrate Cdc25C in rat kangaroo kidney-derived PTK cells (75% inhibition at 6.3 μM). BTO-1 treatment of cultured cells results in mitosis defects consistent with loss-of-Plk1 phenotypes seen in Plk1 RNAi-treated U20S cells, including the appearance of monopolar spindles and the reduction of γ-tubulin at the centrosomes. Plk1 inhibition by BTO-1 in HeLa cells results in a blockage of Rho and Rho-GEF recruitment, which is essential for the assembly of a functional contractile ring.

a benzothiazolo-N-oxide Plk1 inhibitor

Specifications & Purity: BTO-1 is a cell-permeable benzothiazolo-N-oxide compound that targets the ATP-binding pocket of polo-like kinase and is shown to inhibit Plk1 kinase activity in cell-free kinase assays (IC|50|= 8.0 μM) and suppress the phosphorylation of cellular Plk1 substrate Cdc25C in rat kangaroo kidney-derived PTK cells (75% inhibition at 6.3 μM). BTO-1 treatment of cultured cells results in mitosis defects consistent with loss-of-Plk1 phenotypes seen in Plk1 RNAi-treated U20S cells, including the appearance of monopolar spindles and the reduction of γ-tubulin at the centrosomes. Plk1 inhibition by BTO-1 in HeLa cells results in a blockage of Rho and Rho-GEF recruitment, which is essential for the assembly of a functional contractile ring.

Grade: Moligand™

Molecular Formula: C9H4N4O4S

Molecular Weight: 264.22

EC Number: 637-022-0

PubChem CID: 9548007

Isomeric SMILES: C1=C(C=C(C2=C1[N+](=C(S2)C(=O)N)[O-])[N+](=O)[O-])C#N

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

More Information: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/B338594-SCI_b8f273700b3c394d869f6b28aec5ab8a.pdf

UPC:

51132227

Weight:

1.09 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

B338594-5mg

Country of Origin:

China

CAS:

40647-02-7

Product Size:

5mg

Harmonized Tariff Code:

2931.90.90

Hazard Statement Codes:

H302;H312;H315;H319;H332;H335

Precautionary Statement Codes:

P261;P264;P270;P271;P280;P321;P330;P302+P352;P304+P340;P305+P351+P338;P362+P364;P405;P403+P233;P501;P264+P265;P301+P317;P317;P337+P317;P332+P317;P319