Cell Line Origin
Mouse hepatoma
Cell Line Description
A mouse hepatoma isolated from the BW7756 transplantable hepatoma carried in C57 leaden/J mice (see reference: Differentiation). Will synthesise in appreciable amounts only hepatic proteins that appear early in foetal development. The majority of cells contain at least two and often several nucleoli, fat droplets are rare. A mixture of BW1-J and Fao cells were treated with inactive Sendai Virus to produce hybrids at 1 hybrid colony/50 BW1-J cells.
Culture Medium
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)
Subculture Routine
Split semi-confluent cultures (70%) 1:50 i.e. seeding at 2x1000 - 5x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Media change three times a week.
Other Notes
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biological source: mouse skin. growth mode: Adherent. karyotype: Hypotetraploid. morphology: Epithelial. products: Alpha-foetoprotein and Albumin. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94101901-1VL