HTC RAT HEPATOMA (C005B-237570)

Cell Line Origin

Rat hepatoma

Cell Line Description

Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.

Application

Tumourigenicity and enzymatic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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biological source: rat liver. growth mode: Adherent. karyotype: 2n : 66, hypertriploid. morphology: Epithelial. products: Not specified. receptors: Dexamethasone phosphate. hydrocortisone. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:

51201815

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

93120108-1VL

Temperature Control Device:

Yes