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Sigma-Aldrich

CALCIUM CHLORIDE DIHYDRATE MOLECULAR BIOLOGY REAGENT >=99.0% (C005B-003111)

Application Calcium chloride dihydrate has been used: to prepare competent E. coli for transformation(118) in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119) as a component of renaturation buffer for the

Description

Application

Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Biochem/physiol Actions

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.

Specifications
UPC:
51286203
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
100
Unit of Measurement:
GR
MPN:
C3306-100G
CAS:
10035-04-8
Catalog No. C005B-003111
Price: $102.68
List Price: $114.09
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