General description
A 17-residue peptide based on the calmodulin-binding domain of myosin light chain kinase (MLCK). Binds to calmodulin with high affinity (Kd = 6 pM).
A 17-residue peptide based on the calmodulin-binding domain of myosin light chain kinase (MLCK). Binds to calmodulin with high affinity (Kd = 6 pM). Inhibits calmodulin activation of MLCK-catalyzed phosphorylation of smooth muscle regulatory light chain(MLC).
Biochem/physiol Actions
Cell permeable: no
Kd = 6 pM in binding to calmodulin
Primary Target
Calmodulin
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Standard Handling (A)
Sequence
Ac-Arg-Arg-Lys-Trp-Gln-Lys-Thr-Gly-His-Ala-Val-Arg-Ala-Ile-Gly-Arg-Leu-NH₂
Physical form
Supplied as a trifluoroacetate salt.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Torok,K., et al. 1998. Biochemistry 37, 6188.
Török, K., and Trentham, D.R. 1994. Biochemistry33, 12807.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 12352202
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 208735-500UG