Caspase 8, recombinant human active

General description

Caspase-8 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. As with other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-8 consists of large and small subunits which associate to form the active enzyme. The active caspase-8 has been shown to be involved in the proteolysis of poly (ADP-ribose) polymerase (PARP), an enzyme that is involved in DNA repair and genomic maintenance.

The active recombinant human caspase-8 was expressed in E. coli. The expressed caspase-8 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme. The recombinant caspase-8 preferentially cleaves the substrates consisting of consensus sequence IETD (e.g., IETD-AFC and IETD-pNA). The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificty of caspase substrates.Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays. Optimal working dilutions must be determined by end user.

Application

Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays.

Optimal working dilutions must be determined by end user.

Unit Definition

Specific Activity: Where one unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM HEPES, 50 mM NaCl, 0.1% CHAPS, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Physical form

Lyophilized. Reconstitute with 25 μl PBS containing 15% glycerol.

Storage and Stability

Maintain at -70°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.