Cell Line Origin
Mouse (CxDBA)F1 melanoma
Cell Line Description
Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.
Application
Cloning studies
Culture Medium
Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: mouse skin. growth mode: Adherent. karyotype: Hypertetraploid. morphology: Epithelial. products: Melanin. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 87081806-1VL