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Sigma-Aldrich

CLONE M-3 MOUSE (CXDBA)F1 MELANOMA (C005B-235171)

Cell Line Origin Mouse (CxDBA)F1 melanoma Cell Line Description Established from a Cloudman S91 melanoma in a male mouse the cells produce melanin. Application Cloning studies Culture Medium Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) +

Description

Cell Line Origin

Mouse (CxDBA)F1 melanoma

Cell Line Description

Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.

Application

Cloning studies

Culture Medium

Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: mouse skin. growth mode: Adherent. karyotype: Hypertetraploid. morphology: Epithelial. products: Melanin. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

Specifications
UPC:
51422318
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
Quantity:
1
Unit of Measurement:
VI
MPN:
87081806-1VL
Temperature Control Device:
Yes
Catalog No. C005B-235171
Price: $1,440.75
List Price: $1,600.83
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