CM3.B6

Cell Line Origin

Human anti-hepatitis C virus IgG1 secreting B cell

Cell Line Description

Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific for non-structural protein NS3 (helicase domain). This antibody binds to a major conformational epitiope located at the carboxyl-terminal end of the NS3 region that is recognised by approximately 90% from HCV-infected patients. CM3.B6 can be used in immunochemistry, HCV protein purification and HCV typing. CM3.B6 and B12.F8 (Sigma product number 11060102) were derived from the same patient as confirmed by STR profiling.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16

Culture Medium

RPMI 1640 (R0883) + 2 mM L-L-Glutamine (G7513) + 1% NEAA (M7145) + 1% NaP (S8636) + 10% FBS / FCS (F2442) + 20U/ml Interlukin-6 (IL-6).

Subculture Routine

Maintain cultures between 3-9 x 10⁵/ml. 5% CO₂; 37 °C. Media change every two days. Saturation density 1.1 x 10⁶/ml

Other Notes

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Disclaimer

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

Shipping Information:

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UPC:

41181520

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

VI

MPN:

11060101-1VL

Temperature Control Device:

Yes

Controlled Substances and Cyanide:

Yes