Collagenase AF-1 From Clostridium Histolyticum (C09-0814-950)

DescriptionCollagenase AF-1 is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often in combination with Neutral Protease AF  is suitable for the dissociation of sensitive cells from several tissues. The animal-free enzymes have been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation and stromal vascular fraction (SVF) from adipose tissue and tumor cells.ApplicationCollagenase AF-1 can be used for several cell isolations. Our bigger packaging size of 2000 U is ideal for human islet isolation, whereas the smaller size of 150 U can be used for tumor cell isolation, isolation of TILs or SVF from adipose tissue. SpecificationsActivity (Wünsch) ≥ 3.00 U/mg*.Endotoxin ≤ 10.0 EU**/mg (Ph. Eur.)Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia.Endproduct is 0.22 µm filtered. (Clostridiopeptidase A, Collagenase A)EC 3.4.24.3 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1]References and Definitions*Unit definitions:Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.**Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)].References1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-30912. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51. Specification: ≥ 3.00 U/mg MDL Number: MFCD00130830 EC Number: 232-582-9 PubChem CID: 44384985 Isomeric SMILES: CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C278962-SCI_2ce14a843d45f9c368904eee2221f663.pdf