Collagenase NB 5 From Clostridium Histolyticum

DescriptionCollagenase NB 5 is a crude collagenase that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation.Collagenase NB 5 is applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.ApplicationCollagenase NB 5 is suitable for dissociation of a broad variety of tissue types. SpecificationsContains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities.Activity (Wünsch): ≥ 0.10 U/mgMicrobiological examination: Sterility, absence of ClostridiaSterile according to Ph. Eur.(Clostridiopeptidase A)EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]References and DefinitionsUnit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.References1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-30912. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51. Specification: Sterile Grade,≥ 0.10 U/mg MDL Number: MFCD00130830 EC Number: 232-582-9 PubChem CID: 44384985 Isomeric SMILES: CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C279009-SCI_fee7d1ab468ecedc6baebdb46c30b272.pdf