CSPD (C008B-270760)

CSPD is a chemiluminescent substrate for alkaline phosphatase, used for blotting proteins or nucleic acids on nitrocellulose membranes. These substrates can also be used in solution-based assays. The CSPD chemiluminescent substrate enables detection of alkaline phosphatase and alkaline phosphatase–labeled molecules with excellent sensitivity, speed, and ease of use. CSPD chemiluminescent substrate provides a highly sensitive alternative to the widely used fluorescent substrate methylumbelliferyl phosphate (MUP) and the colorimetric substrate p-nitrophenyl phosphate (pNPP). The combination of low background emission and high-intensity light output allows detection of alkaline phosphatase labels with the highest possible sensitivity and signal-to-noise ratio. This versatile chemiluminescent substrate demonstrates high sensitivity in membrane-based applications such as Southern, Northern, and Western blotting. It can also be used in solution-based assays, including immunoassays, DNA probe assays, enzyme assays, and reporter gene assays. Maximum light emission is reached in approximately 10 minutes, and glow emission persists for several hours. Solution-based assays require a chemiluminescent enhancer. Membrane-based assays may also require this reagent to increase light output and sensitivity.

Specifications & Purity: CSPD is a chemiluminescent substrate for alkaline phosphatase, used for blotting proteins or nucleic acids on nitrocellulose membranes. These substrates can also be used in solution-based assays. The CSPD chemiluminescent substrate enables detection of alkaline phosphatase and alkaline phosphatase–labeled molecules with excellent sensitivity, speed, and ease of use. CSPD chemiluminescent substrate provides a highly sensitive alternative to the widely used fluorescent substrate methylumbelliferyl phosphate (MUP) and the colorimetric substrate p-nitrophenyl phosphate (pNPP). The combination of low background emission and high-intensity light output allows detection of alkaline phosphatase labels with the highest possible sensitivity and signal-to-noise ratio. This versatile chemiluminescent substrate demonstrates high sensitivity in membrane-based applications such as Southern, Northern, and Western blotting. It can also be used in solution-based assays, including immunoassays, DNA probe assays, enzyme assays, and reporter gene assays. Maximum light emission is reached in approximately 10 minutes, and glow emission persists for several hours. Solution-based assays require a chemiluminescent enhancer. Membrane-based assays may also require this reagent to increase light output and sensitivity.

Grade: Moligand™

Molecular Formula: C18H22ClO7P

Molecular Weight: 416.79

PubChem CID: 64633

Storage Temperature: Protected from light;Store at -20°C;Argon charged

Shipped in: Ice chest + Ice pads

UPC:

51283709

Weight:

1.06 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

C1510850-25mg

Country of Origin:

China

CAS:

142456-88-0

Product Size:

25mg

Harmonized Tariff Code:

2931.90.90