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Sigma-Aldrich

D98/AH2 CLONE BHUMAN CERVICAL CARCINOMA HELA CH (C005B-234512)

Cell Line Origin Human cervix carcinoma Cell Line Description This cell line has been shown to be resistant to 8-azaguanine. The cell line was recloned in the depositor′s laboratory and is useful in transformation studies.

Description

Cell Line Origin

Human cervix carcinoma

Cell Line Description

This cell line has been shown to be resistant to 8-azaguanine. The cell line was recloned in the depositor′s laboratory and is useful in transformation studies. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Application

Transformation studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: human cervix. growth mode: Adherent. karyotype: 2n : 55. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

Specifications
UPC:
41106514
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
Yes
MPN:
85112701-1VL
Temperature Control Device:
Yes
Catalog No. C005B-234512
Price: $1,906.09
List Price: $2,117.88
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