Dehydroascorbic Acid (DHA) Content Assay Kit (Phenanthroline, Colorimetric Method)

Vitamin C, also known as L-Ascorbic Acid (AsA) , is an essential nutrient for higher primates and a few other organisms. In living organisms, Vitamin C acts as an antioxidant; it is an acidic hexose derivative and a lactone of an enolic sugar acid, protecting the body from damage by free radicals. It also functions as a coenzyme. Its widespread dietary sources are various fresh fruits and vegetables. Vitamin C has two isomers, the L-form and the D-form, but only the L-form possesses physiological function. Both the reduced and oxidized forms exhibit physiological activity. The detection principle of this kit is as follows: A reducing agent is used to convert dehydroascorbic acid to reduced ascorbic acid. Under acidic conditions, the reduced ascorbic acid reduces ferric ions (Fe³⁺) to ferrous ions (Fe²⁺), which then form a stable red chelate complex with phenanthroline. The absorbance is measured at 534 nm using a spectrophotometer. Within a specific concentration range (sample concentration controlled between 1-40 μg/ml), the absorbance shows a linear relationship with the ascorbic acid content, allowing for the determination of ascorbic acid concentration. This kit is primarily used for detecting Vitamin C (ascorbic acid) in plant tissues. It measures the total ascorbic acid content; by subtracting the original reduced ascorbic acid content present in the sample, the dehydroascorbic acid content can be obtained. The advantages of this method are: 1. Stable reaction with minimal fading; 2. Simple operation; 3. No interference from reducing sugars or other common reducing substances, ensuring good specificity; 4. High sensitivity. This product is for research use only and is not intended for use in diagnostic procedures.

Specifications & Purity: Vitamin C, also known as L-Ascorbic Acid (AsA) , is an essential nutrient for higher primates and a few other organisms. In living organisms, Vitamin C acts as an antioxidant; it is an acidic hexose derivative and a lactone of an enolic sugar acid, protecting the body from damage by free radicals. It also functions as a coenzyme. Its widespread dietary sources are various fresh fruits and vegetables. Vitamin C has two isomers, the L-form and the D-form, but only the L-form possesses physiological function. Both the reduced and oxidized forms exhibit physiological activity. The detection principle of this kit is as follows: A reducing agent is used to convert dehydroascorbic acid to reduced ascorbic acid. Under acidic conditions, the reduced ascorbic acid reduces ferric ions (Fe³⁺) to ferrous ions (Fe²⁺), which then form a stable red chelate complex with phenanthroline. The absorbance is measured at 534 nm using a spectrophotometer. Within a specific concentration range (sample concentration controlled between 1-40 μg/ml), the absorbance shows a linear relationship with the ascorbic acid content, allowing for the determination of ascorbic acid concentration. This kit is primarily used for detecting Vitamin C (ascorbic acid) in plant tissues. It measures the total ascorbic acid content; by subtracting the original reduced ascorbic acid content present in the sample, the dehydroascorbic acid content can be obtained. The advantages of this method are: 1. Stable reaction with minimal fading; 2. Simple operation; 3. No interference from reducing sugars or other common reducing substances, ensuring good specificity; 4. High sensitivity. This product is for research use only and is not intended for use in diagnostic procedures.

Grade: BioReagent

Storage Temperature: Store at 2-8°C;Protected from light

Shipped in: Wet ice

UPC:

41141618

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

ST

WeightUOM:

LB

MPN:

D1521842-50T

Country of Origin:

China

Product Size:

50T

Harmonized Tariff Code:

2931.90.90