HS F5 5UM 5CMX2.1MM DISCOVERY HPLC COLUMN (C005B-230240)

General description

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Features and Benefits

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

material: stainless steel column. Quality Level: 100. Agency: suitable for USP L43. product line: Discovery^®. . feature: endcapped. manufacturer/tradename: Discovery^®. . packaging: 1 ea of . extent of labeling: 12% Carbon loading. parameter: ≤. 70 °C temp. range, 400 bar pressure (5801 psi). technique(s): HPLC: suitable, LC/MS: suitable. L ƒ I.D.: 5 . cm ƒ 2.1 . mm . surface area: 300 . m²/g . surface coverage: 4 . μ. mol/m². impurities: <. 10 . ppm metals. matrix: silica gel, high purity, spherical particle platform, fully porous particle. matrix active group: PFP (pentafluorophenyl) phase. particle size: 5 . μ. m. pore size: 120 . ƒ.... operating pH range: 2-8. application(s): food and beverages. separation technique: reversed phase.

UPC:

41115709

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

MPN:

567508-U