DL-Dithiothreitol (C007B-555611)

Dithiothreitol (DTT) is a thiol-reducing reagent that quantitatively reduces disulfide (SH) groups in biological molecules. It specifically targets the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is more efficient than that of 2-mercaptoethanol (5% v/v, 700 mM).Application:DTT has been used:as an enzyme stabilizer to maintain exebacase stabilityfor peptide extraction and protein denaturation in sample preparation and reversal of formaldehyde crosslinks before mass spectrometry as a buffer component to extract synaptogliosomeAn excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Specification: BioUltra, for molecular biology, ≥99.5%(RT)

MDL Number: MFCD00004877

Molecular Formula: C4H10O2S2

Molecular Weight: 154.25

EC Number: MFCD00004877

PubChem CID: 446094

Isomeric SMILES: C([C@H]([C@@H](CS)O)O)S

Beilstein Registry Number: 1719760

UPC:

51202304

Condition:

New

Weight:

0.08 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

D755747-5g

CAS:

3483-12-3

Product Size:

5g

Hazard Statement Codes:

H318:H302:H335:H319:H315