DL-Dithiothreitol(DTT)

Dithiothreitol (DTT) is a thiol-reducing reagent that quantitatively reduces disulfide (SH) groups in biological molecules. It specifically targets the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is more efficient than that of 2-mercaptoethanol (5% v/v, 700 mM). An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM). DTT has been used:as an enzyme stabilizer to maintain exebacase stability. for peptide extraction and protein denaturation. in sample preparation and reversal of formaldehyde crosslinks before mass spectrometry. as a buffer component to extract synaptogliosome.

Specifications & Purity: Dithiothreitol (DTT) is a thiol-reducing reagent that quantitatively reduces disulfide (SH) groups in biological molecules. It specifically targets the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is more efficient than that of 2-mercaptoethanol (5% v/v, 700 mM). An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM). DTT has been used:as an enzyme stabilizer to maintain exebacase stability. for peptide extraction and protein denaturation. in sample preparation and reversal of formaldehyde crosslinks before mass spectrometry. as a buffer component to extract synaptogliosome.

Grade: UltraBio™;Suitable for molecular biology

MDL Number: MFCD00004877

Molecular Formula: C4H10O2S2

Molecular Weight: 154.25

EC Number: 222-468-7

PubChem CID: 446094

Isomeric SMILES: C([C@H]([C@@H](CS)O)O)S

Beilstein: 1719760

Storage Temperature: Store at 2-8°C

Shipped in: Wet ice

UPC:

51201631

Weight:

1.15 Ounces

HazmatClass:

Yes

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

D755747-250mg

Country of Origin:

China

CAS:

3483-12-3

Product Size:

250mg

Harmonized Tariff Code:

2931.90.90

Health Hazards:

Corrosive

Hazard Statement Codes:

H302;H315;H318;H319;H335

Precautionary Statement Codes:

P261;P264;P270;P271;P280;P321;P330;P302+P352;P304+P340;P305+P351+P338;P362+P364;P405;P403+P233;P501;P264+P265;P301+P317;P305+P354+P338;P317;P337+P317;P332+P317;P319