DM1500 DNA Marker (C008B-267688)

DNA Marker consists of purified double-stranded DNA of various molecular weights and is a ready-to-use molecular weight standard. The molecular weight and concentration of samples can be estimated based on the position and brightness of bands after electrophoresis. This product is pre-mixed with loading buffer and can be directly loaded for electrophoresis. In addition to verification by agarose electrophoresis, most bands have undergone strict PAGE testing, with no issues such as "double bands" or multiple bands for DNA molecules of the same size observed in PAGE gels. When agarose gels contain macromolecular dyes (e.g., GelRed), adding an electrophoresis enhancer (main component: macromolecular nucleic acid dye) can effectively reduce band smearing of the Marker during electrophoresis. Aladdin GelRed-prestained series products are also available for selection. Experimental procedures:Add 5 μL of Marker to the agarose gel well (5 mm) for electrophoresis; the loading volume can be adjusted according to the well size.Precautions:"Band smearing" is a common phenomenon during electrophoresis when macromolecular dyes (e.g., GelRed) are added to agarose gels. It often manifests as relatively bright low-molecular-weight bands, relatively dim high-molecular-weight bands, overall band sagging, and adhesion between bands. Our experiments reveal that the root cause of this phenomenon is insufficient dye concentration in the gel.Unlike small-molecular-weight water-soluble dyes such as EB, GelRed has extremely low solubility in water, and the recommended working concentration of commercially available GelRed differs greatly from that of EB. Good electrophoresis results are generally obtained when a GelRed-containing gel is first used. However, if the gel is reused or soaked in electrophoresis buffer for too long, the dye in the gel leaches into the buffer, further reducing the dye concentration in the gel. Low-molecular-weight DNA bands enter the gel first and bind to the dye, leaving insufficient dye for subsequent high-molecular-weight DNA, resulting in "band smearing".Two Approaches to Improve "Band Smearing" in Agarose Gel Electrophoresis (Containing Macromolecular Nucleic Acid Dyes)Increase the relative dye concentrationReduce dye loss⁕ Add electrophoresis enhancer (main component: nucleic acid dye) to the Marker in advance.⁕ Increase the dye concentration in the gel (e.g., use 10000× dye at 5000×).⁕ Reduce the loading volume of DNA Marker.⁕ Avoid repeated use of the gel (dye migrates upward out of the gel during electrophoresis).⁕ Prevent prolonged soaking of the gel in electrophoresis buffer to avoid dye leaching.

Specifications & Purity: DNA Marker consists of purified double-stranded DNA of various molecular weights and is a ready-to-use molecular weight standard. The molecular weight and concentration of samples can be estimated based on the position and brightness of bands after electrophoresis. This product is pre-mixed with loading buffer and can be directly loaded for electrophoresis. In addition to verification by agarose electrophoresis, most bands have undergone strict PAGE testing, with no issues such as "double bands" or multiple bands for DNA molecules of the same size observed in PAGE gels. When agarose gels contain macromolecular dyes (e.g., GelRed), adding an electrophoresis enhancer (main component: macromolecular nucleic acid dye) can effectively reduce band smearing of the Marker during electrophoresis. Aladdin GelRed-prestained series products are also available for selection. Experimental procedures:Add 5 μL of Marker to the agarose gel well (5 mm) for electrophoresis; the loading volume can be adjusted according to the well size.Precautions:"Band smearing" is a common phenomenon during electrophoresis when macromolecular dyes (e.g., GelRed) are added to agarose gels. It often manifests as relatively bright low-molecular-weight bands, relatively dim high-molecular-weight bands, overall band sagging, and adhesion between bands. Our experiments reveal that the root cause of this phenomenon is insufficient dye concentration in the gel.Unlike small-molecular-weight water-soluble dyes such as EB, GelRed has extremely low solubility in water, and the recommended working concentration of commercially available GelRed differs greatly from that of EB. Good electrophoresis results are generally obtained when a GelRed-containing gel is first used. However, if the gel is reused or soaked in electrophoresis buffer for too long, the dye in the gel leaches into the buffer, further reducing the dye concentration in the gel. Low-molecular-weight DNA bands enter the gel first and bind to the dye, leaving insufficient dye for subsequent high-molecular-weight DNA, resulting in "band smearing".Two Approaches to Improve "Band Smearing" in Agarose Gel Electrophoresis (Containing Macromolecular Nucleic Acid Dyes)Increase the relative dye concentrationReduce dye loss⁕ Add electrophoresis enhancer (main component: nucleic acid dye) to the Marker in advance.⁕ Increase the dye concentration in the gel (e.g., use 10000× dye at 5000×).⁕ Reduce the loading volume of DNA Marker.⁕ Avoid repeated use of the gel (dye migrates upward out of the gel during electrophoresis).⁕ Prevent prolonged soaking of the gel in electrophoresis buffer to avoid dye leaching.

Grade: BioReagent;Ready-to-use;Suitable for molecular biology;for NA electrophoresis;for PAGE

Storage Temperature: Store at -20°C;Avoid repeated freezing and thawing

Shipped in: Ice chest + Ice pads

UPC:

41105335

Weight:

1.09 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

D1521306-500μl

Country of Origin:

China

Product Size:

500μl

Harmonized Tariff Code:

2931.90.90