DNase I-ST

This product is recombinant DNase I. Compared with the wild-type enzyme, it has been engineered to exhibit significantly enhanced salt tolerance, capable of completely digesting DNA in solutions containing 300 mM monovalent salt. This product is free of RNase contamination.Components ListD1522668ComponentsAppearance1KUStorageD1522668ADNase I-ST (2 U/μl)Liquid500μl-20℃. Avoid freeze/thaw cycles.D1522668B10× DNase I-ST BufferLiquid1.25ml-20℃. Avoid freeze/thaw cycles.Activity DefinitionOne unit (U) is defined as the amount of enzyme required to completely degrade 1 μg of pUC19 plasmid DNA in 10 minutes at 37℃.ApplicationsIn vitro transcription: Removal of template DNA after RNA polymerase-mediated in vitro transcription.Removal of genomic DNA contamination from RNA samples.DNase I footprinting for analyzing DNA-protein interactions.Nick translation labeling of DNA in combination with DNA Polymerase I.Construction of random DNA fragment libraries.Partial digestion of genomic DNA as a positive control in the TUNEL assay for apoptosis detection.Quality ControlProtein PurityDetermined by SDS-PAGE, the protein purity is ≥90%.RNase Residue Detection2 U of DNase I-ST is incubated with 500 ng of total RNA at 37℃ for 1 h. More than 90% of the RNA remains intact as determined by agarose gel electrophoresis.Protocol1. Removal of Genomic DNA Contamination from RNA SamplesReaction Setup:ComponentVolumeRNA1 μg10× DNase I-ST Buffer1 μlDNase I-ST (2 U/μl)0.5 μlNuclease-Free Waterup to 10 μlReaction Conditions: 37℃ for 15 min.Inactivation: Column purification or phenol/chloroform extraction.2. Removal of Template DNA after In Vitro TranscriptionProcedure:① Add 1 U of DNase I-ST per 1 μg of template DNA in the transcription reaction. The enzyme dosage can be optimized as needed. ② Reaction Conditions: 37℃ for 15 min. ③ Inactivation: Column purification or phenol/chloroform extraction.NotesPerform all operations on RNA samples in RNase-free tubes.When using this product to remove DNA from RNA samples, Murine RNase Inhibitor can be added to the reaction system at a final concentration of 1 U/μl to protect RNA from degradation.DNase I-ST is sensitive to physical denaturation. Do not vortex vigorously when mixing.For your safety and health, please wear a lab coat, disposable gloves, and a face mask during the experiment.

Specifications & Purity: This product is recombinant DNase I. Compared with the wild-type enzyme, it has been engineered to exhibit significantly enhanced salt tolerance, capable of completely digesting DNA in solutions containing 300 mM monovalent salt. This product is free of RNase contamination.Components ListD1522668ComponentsAppearance1KUStorageD1522668ADNase I-ST (2 U/μl)Liquid500μl-20℃. Avoid freeze/thaw cycles.D1522668B10× DNase I-ST BufferLiquid1.25ml-20℃. Avoid freeze/thaw cycles.Activity DefinitionOne unit (U) is defined as the amount of enzyme required to completely degrade 1 μg of pUC19 plasmid DNA in 10 minutes at 37℃.ApplicationsIn vitro transcription: Removal of template DNA after RNA polymerase-mediated in vitro transcription.Removal of genomic DNA contamination from RNA samples.DNase I footprinting for analyzing DNA-protein interactions.Nick translation labeling of DNA in combination with DNA Polymerase I.Construction of random DNA fragment libraries.Partial digestion of genomic DNA as a positive control in the TUNEL assay for apoptosis detection.Quality ControlProtein PurityDetermined by SDS-PAGE, the protein purity is ≥90%.RNase Residue Detection2 U of DNase I-ST is incubated with 500 ng of total RNA at 37℃ for 1 h. More than 90% of the RNA remains intact as determined by agarose gel electrophoresis.Protocol1. Removal of Genomic DNA Contamination from RNA SamplesReaction Setup:ComponentVolumeRNA1 μg10× DNase I-ST Buffer1 μlDNase I-ST (2 U/μl)0.5 μlNuclease-Free Waterup to 10 μlReaction Conditions: 37℃ for 15 min.Inactivation: Column purification or phenol/chloroform extraction.2. Removal of Template DNA after In Vitro TranscriptionProcedure:① Add 1 U of DNase I-ST per 1 μg of template DNA in the transcription reaction. The enzyme dosage can be optimized as needed. ② Reaction Conditions: 37℃ for 15 min. ③ Inactivation: Column purification or phenol/chloroform extraction.NotesPerform all operations on RNA samples in RNase-free tubes.When using this product to remove DNA from RNA samples, Murine RNase Inhibitor can be added to the reaction system at a final concentration of 1 U/μl to protect RNA from degradation.DNase I-ST is sensitive to physical denaturation. Do not vortex vigorously when mixing.For your safety and health, please wear a lab coat, disposable gloves, and a face mask during the experiment.

Grade: Recombinant;Suitable for molecular biology;EnzymoPure™;RNase free

Storage Temperature: Store at -20°C;Avoid repeated freezing and thawing

Shipped in: Ice chest + Ice pads

UPC:

12352204

Weight:

7.06 Ounces

HazmatClass:

No

Quantity:

1

Unit of Measurement:

EA

WeightUOM:

LB

MPN:

D1522668-1KU

Country of Origin:

China

Product Size:

1KU

Harmonized Tariff Code:

2931.90.90