DUOLINK(TM) FLOWPLA COMPENSATION BEADS - GREEN

Description

Application

Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

It is recommended that Duolink® compensation beads should be used when performing a multiplexed experiment (DUO96001) to save on time and reagent cost, rather than using precious sample. Please note that these Green beads are specific to the Green fluor utilized in the Duolink® Multicolor FlowPLA kit, not the Green fluorophore in the stand-alone Duolink® microscopy and flow kits. This product can be used to determine flow cytometry instrumentation collection parameters, inform experimental design, and as a control for compensation data for multiplexed FlowPLA experiments.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

Duolink® Multicolor flow PLA Reagent Pack Kits used with Duolink® PLA Multicolor Probemaker Kits will enable Multiplexing of up to 4 protein events with sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed and permeabilized suspended cells, and at least two primary antibodies that specifically recognize your proteins of interest. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension and primary antibodies.

Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). Flow validated antibodies are recommended.

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Features and Benefits

Save time and reagent cost by utilizing beads for compensation rather than precious sample and multiplex reagents

No overexpression or genetic manipulation required

High specificity (fewer false positives)

Single molecule sensitivity due to rolling circle amplification

Relative quantification possible

No special equipment needed

Quicker and simpler than FRET

Increased accuracy compared to co-IP

Publication-ready results

Components

This product is comprised of the following:

6.0 - 8.0μm polystyrene beads suitable for use in flow cytometry assays. The bead slurry should have a concentration of 1.5-3.0 million beads/mL. Green beads can be mixed with the negative control compensation (DUO84010), or collected alone.

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

UPC:

41116015

Condition:

New

Weight:

1.00 Ounces

HazmatClass:

No

Quantity:

3

Unit of Measurement:

ML

MPN:

DUO84012-2.5ML

Temperature Control Device:

Yes