Cell Line Origin
Not specified
Cell Line Description
The ED17 hybridoma cell line secretes antibodies reactive against a 55 kDa membrane antigen on tissue macrophages. ED17 reacts strongly with red pulp macrophages in the spleen and Langerhans cells in the skin as well as peritoneal macrophages. The antibody also recognises rat SIRP (personal communication).
Immunogen
Wistar rat spleen
Application
Immunohistochemistry, cytochemistry, FACS
Culture Medium
RPMI 1640 + 2 mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: Mouse spleen cells. Myeloma: Sp2/0. Reactivity: Anti-myeloid antigen 55kDa, rat. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51131710
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95110627-1VL